Premium
Psoriatic T cells reduce epidermal turnover time and affect cell proliferation contributed from differential gene expression
Author(s) -
Li Junqin,
Li Xinhua,
Hou Ruixia,
Liu Ruifeng,
Zhao Xincheng,
Dong Feng,
Wang Chunfang,
Yin Guohua,
Zhang Kaiming
Publication year - 2015
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12961
Subject(s) - affect (linguistics) , gene expression , cell growth , microbiology and biotechnology , gene , differential (mechanical device) , epidermis (zoology) , biology , cancer research , genetics , psychology , anatomy , communication , physics , thermodynamics
Psoriasis is mediated primarily by T cells, which reduce epidermal turnover time and affect keratinocyte proliferation. We aimed to identify differentially expressed genes ( DEG ) in T cells from normal, five pairs of monozygotic twins concordant or discordant for psoriasis, to determine whether these DEG may account for the influence to epidermal turnover time and keratinocyte proliferation. The impact of T cells on keratinocyte proliferation and epidermal turnover time were investigated separately by immunohistochemistry and cultured with 3 H‐TdR. mRNA expression patterns were investigated by RNA sequencing and verified by real‐time reverse transcription polymerase chain reaction. After co‐culture with psoriatic T cells, the expression of Ki‐67, c‐Myc and p53 increased, while expression of Bcl‐2 and epidermal turnover time decreased. There were 14 DEG which were found to participate in the regulation of cell proliferation or differentiation. Psoriatic T cells exhibited the ability to decrease epidermal turnover time and affect keratinocyte proliferation because of the differential expression of PPIL 1 , HSPH 1 , SENP 3 , NUP 54 , FABP 5 , PLEKHG 3 , SLC 9A9 and CHCHD 4 .