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Mating type gene for isolates of Trichophyton mentagrophytes from guinea pigs
Author(s) -
Hiruma Junichiro,
Kano Rui,
Kimura Utako,
Takamori Kenji,
Suga Yasushi,
Hiruma Masataro,
Hasegawa Atsuhiko,
Tsuboi Ryoji
Publication year - 2014
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12575
Subject(s) - trichophyton , biology , guinea pig , gene , polymerase chain reaction , mating type , population , strain (injury) , microbiology and biotechnology , dermatophyte , mating , cavia , genetics , medicine , anatomy , antifungal , environmental health
Trichophyton mentagrophytes were isolated from 19 of 20 guinea pigs in a children's corner of a zoo. The nucleotide sequence identity of the internal transcribed spacer region among 19 guinea pig isolates was 99%, including the reference strain of animal type 3 of T. mentagrophytes . The genomic DNA of all isolates were investigated for the mating ( MAT ) gene by specific polymerase chain reaction. The alpha‐box gene was detected in all 19 isolates, while the high‐mobility‐group ( HMG ) gene was detected in only one of 19 isolates. Therefore, the guinea pig population harbored at least 2 MAT types of Arthroderma vanbreuseghemii . The T. mentagrophytes that was prevalent in this population may constitute a constant source of infection for persons coming into contact with guinea pigs.