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Biologic therapy improves psoriasis by decreasing the activity of monocytes and neutrophils
Author(s) -
Yamanaka Keiichi,
Umezawa Yoshinori,
Yamagiwa Akisa,
Saeki Hidehisa,
Kondo Makoto,
Gabazza Esteban C.,
Nakagawa Hidemi,
Mizutani Hitoshi
Publication year - 2014
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12560
Subject(s) - psoriasis , medicine , immunology , tumor necrosis factor alpha , cytokine , immunosuppression , monoclonal antibody , immune system , monocyte , immunotherapy , antibody
Therapy with monoclonal antibodies to tumor necrosis factor ( TNF )‐α and the interleukin ( IL )‐12/23 p40 subunit has significantly improved the clinical outcome of patients with psoriasis. These antibodies inhibit the effects of the target cytokines and thus the major concern during their use is the induction of excessive immunosuppression. Recent studies evaluating the long‐term efficacy and safety of biologic therapy in psoriasis have shown no significant appearance of serious adverse effects including infections and malignancies. However, the immunological consequence and the mechanism by which the blockade of a single cytokine by biologics can successfully control the activity of psoriasis remain unclear. In the current study, we investigated the effect of biologic therapy on cytokine production of various lymphocytes and on the activity of monocytes and neutrophils in psoriatic patients. Neutrophils, monocytes and T cells were purified from heparinized peripheral venous blood by Ficoll density gradient centrifugation, and γ‐interferon, TNF ‐α and IL ‐17 production from lymphocytes was measured by flow cytometer. The activation maker of neutrophils and the activated subsets of monocytes were also analyzed. Biologic therapy induced no significant changes in the cytokine production by lymphocytes from the skin and gut‐homing T cells. However, neutrophil activity and the ratio of activated monocyte population increased in severely psoriatic patients were normalized in psoriatic patients receiving biologic therapy. The present study showed that biologic therapy ameliorates clinical symptoms and controls the immune response in patients with psoriasis.