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Utilization of matrix‐assisted laser desorption and ionization time‐of‐flight mass spectrometry for identification of infantile seborrheic dermatitis‐causing M alassezia and incidence of culture‐based cutaneous M alassezia microbiota of 1‐month‐old infants
Author(s) -
Yamamoto Mikachi,
Umeda Yoshiko,
Yo Ayaka,
Yamaura Mariko,
Makimura Koichi
Publication year - 2014
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12364
Subject(s) - seborrheic dermatitis , mass spectrometry , dermatology , identification (biology) , incidence (geometry) , time of flight mass spectrometry , desorption , medicine , ionization , chemistry , chromatography , physics , biology , optics , ion , organic chemistry , adsorption , botany
Matrix‐assisted laser desorption and ionization time‐of‐flight mass spectrometry ( MALDI ‐ TOF ‐ MS ) has been utilized for identification of various microorganisms. M alassezia species, including M alassezia restricta , which is associated with seborrheic dermatitis, has been difficult to identify by traditional means. This study was performed to develop a system for identification of M alassezia species with MALDI ‐ TOF ‐ MS and to investigate the incidence and variety of cutaneous M alassezia microbiota of 1‐month‐old infants using this technique. A M alassezia species‐specific MALDI ‐ TOF ‐ MS database was developed from eight standard strains, and the availability of this system was assessed using 54 clinical strains isolated from the skin of 1‐month‐old infants. Clinical isolates were cultured initially on CHROMagar M alassezia growth medium, and the 28S ribosomal DNA (D1/D2) sequence was analyzed for confirmatory identification. Using this database, we detected and analyzed M alassezia species in 68% and 44% of infants with and without infantile seborrheic dermatitis, respectively. The results of MALDI ‐ TOF ‐ MS analysis were consistent with those of r DNA sequencing identification (100% accuracy rate). To our knowledge, this is the first report of a MALDI ‐ TOF ‐ MS database for major skin pathogenic M alassezia species. This system is an easy, rapid and reliable method for identification of M alassezia .