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Identification of Wnt/β‐catenin signaling pathway in dermal papilla cells of human scalp hair follicles: TCF 4 regulates the proliferation and secretory activity of dermal papilla cell
Author(s) -
Xiong Ya,
Liu Yi,
Song ZhiQiang,
Hao Fei,
Yang XiChuan
Publication year - 2014
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12313
Subject(s) - wnt signaling pathway , dermal papillae , hair follicle , microbiology and biotechnology , transfection , biology , hair cycle , cell growth , signal transduction , cell culture , genetics
It is clear that the dermal papilla cell ( DPC ), which is located at the bottom of the hair follicle, is a special mesenchymal component, and it plays a leading role in regulating hair follicle development and periodic regeneration. Recent studies showed that the Wnt signaling pathway through β‐catenin (canonical Wnt signaling pathway) is an essential component in maintaining the hair‐inducing activity of the dermal papilla and growth of hair papilla cells. However, the intrinsic pathways and regulating mechanism are largely unknown. In the previous work, we constructed a c DNA subtractive library of DPC and first found that the TCF 4 gene, as a key factor of Wnt signaling pathway, was expressed as the upregulated gene of the hair follicle in low‐passage DPC . This study was to explore the role of TCF 4 in regulating the proliferation and secretory activity of DPC . We constructed a pc DNA 3.0– TCF 4 expression vector and transfected it into DPC to achieve stable expression by bangosome 2000. Furthermore, we used the method of chemosynthesis to synthesize three pairs of TCF 4 si RNA and transfected them into DPC . Meanwhile, we compared the transfection group and non‐transfection group. We first proposed that there was expression difference in TCF 4 in DPC under different biological condition. This study may have a high impact on the molecular mechanism of follicular lesions and provide a new vision for the treatment of clinic diseases.