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Expression analysis of ovostatin 2 reveals its involvement in proliferation, invasion and angiogenesis of cutaneous malignant melanoma
Author(s) -
Huang YingXue,
Qi Jinliang,
Wang HongSheng,
Shao XueBao,
Zeng XueSi,
Li AMei,
Xu XiuLian,
Sun JianFang
Publication year - 2013
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12294
Subject(s) - melanoma , immunohistochemistry , western blot , pathology , angiogenesis , staining , biology , vascular endothelial growth factor , h&e stain , medicine , cancer research , vegf receptors , biochemistry , gene
The relationship of ovostatin 2 ( OVOS 2) expression with the clinicopathological features of cutaneous malignant melanoma ( CMM ) was investigated to identify OVOS 2 expression in cutaneous melanocytic lesions, and to reveal whether OVOS 2 has a function in melanoma progression. Eight specimens of CMM and paracancerous tissue were analyzed using real‐time polymerase chain reaction ( PCR ) and western blot for the m RNA and protein expression of OVOS 2, respectively. Immunohistochemical staining was performed on 52 CMM and 62 nevi, followed by clinicopathological significance analysis. The proliferative cells were visualized by staining with Ki‐67 antibody. The intensity of angiogenesis was assessed by staining with vascular endothelial growth factor ( VEGF ). Real‐time PCR and western blot analyses showed that OVOS 2 was significantly upregulated in cutaneous melanoma than in paired normal skins. Immunohistochemistry showed that 86.5% (45/52) of malignant cases showed OVOS 2 cytoplasmic expression compared with 29% (18/62) in benign nevi. OVOS2 expression was significantly higher in invasive and metastatic melanoma than in in situ melanoma ( P < 0.01). Furthermore, OVOS 2 expression was positively correlated with the known prognostic variables of melanoma including clinical stage, C lark level and B reslow depth. It was also significantly associated with ulcer status, Ki‐67 labeling index and VEGF expression in primary melanoma. OVOS 2 expression was significantly increased in CMM , which increased incrementally from benign nevi to melanoma and appeared to be involved in the progression of melanoma.