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Immunohistochemical characterization of non‐epithelial cells in spiradenoma
Author(s) -
Iida Kohji,
Iwai Shinsaku,
Hosaka Hiroomi,
Kitami Yuki,
Akiyama Masaki,
Suzuki Takao,
Sueki Hirohiko
Publication year - 2013
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12264
Subject(s) - parenchyma , immunohistochemistry , pathology , immunofluorescence , epithelium , biology , chemistry , antibody , medicine , immunology
Spiradenoma is unique with respect to the presence of a large number of non‐epithelial cells, including S100 protein + cells, most of which are presumably L angerhans cells, in the parenchyma as shown in the published work. However, the characterization of these non‐epithelial cells to date is insufficient. Immunohistochemistry of CD 1a, CD 3, CD 4, CD 8, CD 56, CD 68, intercellular adhesion molecule‐1 ( ICAM ‐1), and HLA ‐ DR , as well as double‐immunofluorescence labeling of S100 protein/ CD 1a and CD 1a/ CD 3, was performed using paraffin‐embedded specimens from five cases of spiradenoma retrospectively. Non‐epithelial cells evenly distributed throughout the parenchyma of spiradenoma primarily consisted of CD 1a + L angerhans cells and CD 3 + T cells. ICAM ‐1 was expressed by epithelial cells and non‐epithelial cells in the parenchyma. HLA ‐ DR on the epithelial cells was limited to the focal area. In double‐immunofluorescence labeling, approximately one‐half of L angerhans cells were spatially related to T cells in the parenchyma, suggesting their functional interaction.