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In vitro responses of neurofibroma fibroblasts, mast cells and S chwann cells obtained from patients with neurofibromatosis 1 to 308‐nm excimer light and/or vitamin D 3
Author(s) -
Nakayama Juichiro,
Sato Chiemi,
Imafuku Shinichi
Publication year - 2013
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12242
Subject(s) - neurofibroma , neurofibromatosis , in vitro , excimer , in vivo , irradiation , cancer research , microbiology and biotechnology , chemistry , pathology , medicine , biology , fluorescence , biochemistry , optics , physics , nuclear physics
Fibroblasts, mast cells and S chwann cells were isolated from neurofibromas of patients with neurofibromatosis 1, and their responses to 308‐nm excimer light irradiation and/or vitamin D 3 or an analog (tacalcitol; 1,24‐dihydroxyvitamin D 3 ) were examined in vitro . Excimer light irradiation (300 mJ/cm 2 ) suppressed the growth of all three cell types. Exposure to 10 − 7 mol/L of 1α,25( OH ) 2 D 3 ( VD 3 ) or tacalcitol suppressed the growth of fibroblasts and mast cells, but not Schwann cells. These results suggest that the different neurofibroma cell types show different responses to VD 3 . A combination of excimer light irradiation and VD 3 is necessary to suppress the growth of neurofibroma cells in vivo .