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Urease gene of T richophyton rubrum var. raubitschekii
Author(s) -
HIRUMA Midori,
KANO Rui,
SUGITA Takashi,
MOCHIZUKI Takashi,
HASEGAWA Atsuhiko,
HIRUMA Masataro
Publication year - 2013
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.12017
Subject(s) - trichophyton rubrum , gene , urease , biology , genbank , microbiology and biotechnology , biochemistry , enzyme , antifungal
The recent description (in G en B ank) of a urease‐encoding gene from T richophyton rubrum provides an opportunity to compare this species to the highly similar T . rubrum var. raubitschekii . Therefore, the corresponding genomic DNA was recovered from T . rubrum var. raubitschekii , and the sequence and expression were compared for this urease gene in urease‐positive and ‐negative isolates of T . rubrum and T . rubrum var. raubitschekii . The sequence (2371 bp) of the T . rubrum var. raubitschekii urease gene revealed the presence of three exons. Except for a three‐amino acid insertion, the predicted proteins were identical, but demonstrated protein identity of approximately 70% compared to an Arthroderma gypseum homolog. Reverse transcription polymerase chain reaction analysis revealed the presence of a corresponding transcript in both urease‐positive T . rubrum var. raubitschekii isolates examined; the same analysis did not detect this transcript in urease‐negative isolates of T . rubrum .