Metabolic modeling identifies determinants of thermal growth responses in Arabidopsis thaliana

Summary Temperature is a critical environmental factor affecting nearly all plant processes, including growth, development, and yield. Yet, despite decades of research, we lack the ability to predict plant performance at different temperatures, limiting the development of climate‐resilient crops. Further, there is a pressing need to bridge the gap between the prediction of physiological and molecular traits to improve our understanding and manipulation of plant temperature responses. Here, we developed the first enzyme‐constrained model of Arabidopsis thaliana 's metabolism, facilitating predictions of growth‐related phenotypes at different temperatures. We showed that the model can be employed for in silico identification of genes that affect plant growth at suboptimal growth temperature. Using mutant lines, we validated the genes predicted to affect plant growth, demonstrating the potential of metabolic modeling in accurately predicting plant thermal responses. The temperature‐dependent enzyme‐constrained metabolic model provides a template that can be used for developing sophisticated strategies to engineer climate‐resilient crops.