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Identification and characterisation of temporal abundance of microRNAs in synovial fluid from an experimental equine model of osteoarthritis
Author(s) -
Walters Marie,
Skovgaard Kerstin,
Heegaard Peter M. H.,
Fang Yongxiang,
Kharaz Yalda A.,
Bundgaard Louise,
Skovgaard Lene T.,
Jensen Henrik E.,
Andersen Pia H.,
Peffers Mandy J.,
Jacobsen Stine
Publication year - 2025
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.1111/evj.14456
Subject(s) - microrna , osteoarthritis , biology , synovial fluid , gene expression , gene , rna extraction , rna , bioinformatics , computational biology , genetics , pathology , medicine , alternative medicine
Abstract Background MicroRNAs, a class of small noncoding RNAs, serve as post‐transcriptional regulators of gene expression and are present in a stable and quantifiable form in biological fluids. MicroRNAs may influence intra‐articular responses and the course of disease, but very little is known about their temporal changes in osteoarthritis. Objectives To identify miRNAs and characterise the temporal changes in their abundance in SF from horses with experimentally induced osteoarthritis. We hypothesised that the abundance of miRNA would change during disease progression. Study design In vivo experiments. Methods RNA extracted from synovial fluid obtained sequentially (Day 0, 28 and 70) from nine horses with experimentally induced osteoarthritis was subjected to small RNA sequencing using the Illumina Hiseq 4000 sequencing platform. Differentially abundant miRNAs underwent further validation and mapping of temporal abundance (Day 0, 14, 17, 21, 28, 35, 42, 49, 56, 63 and 70 days after osteoarthritis induction) by microfluidic reverse transcription quantitative real‐time PCR. Bioinformatic analyses were performed to predict potential biological associations and target genes of the differentially abundant microRNAs. Results Small RNA sequencing revealed 61 differentially abundant microRNAs at an early osteoarthritis stage (Day 28), and subsequent reverse transcription quantitative real‐time PCR analysis validated 20 of these. Significant biological functions of the differentially abundant microRNAs were apoptosis, necrosis, cell proliferation and cell invasion. Following validation, four microRNAs (miRNA‐199b‐3p, miRNA‐139‐5p, miRNA‐1839 and miRNA‐151‐5p) were detected in more than 50% of the synovial fluid samples and had higher abundance in osteoarthritic than in control joints. Main limitations Limited sample size. Conclusion This is the first study to determine longitudinal changes in synovial fluid microRNA abundance in an equine model of osteoarthritis. Larger studies are needed in naturally occurring osteoarthritis to interrogate putative changes identified by this study.