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For the record: Temperature‐sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein
Author(s) -
Blance Stephen J.,
Williams Nicola L.,
Preston ZOË A.,
Bishara John,
Smyth Michael S.,
Maxwell Anthony
Publication year - 2000
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.9.5.1035
Subject(s) - dna gyrase , escherichia coli , mutation , suppressor , suppressor mutation , genetics , dna , biology , strain (injury) , phenotype , microbiology and biotechnology , gene , anatomy
Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second‐site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon structural considerations.