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Detection of intact megaDalton protein assemblies of vanillyl‐alcohol oxidase by mass spectrometry
Author(s) -
Van Berkel Willem J. H.,
Van Den Heuvel Robert H. H.,
Versluis Cees,
Heck Albert J. R.
Publication year - 2000
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.9.3.435
Subject(s) - mass spectrometry , chemistry , electrospray ionization , chromatography , electrospray , analytical chemistry (journal) , molecular mass , enzyme , biochemistry
Well‐resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2%. the mass spectral data seem to reflect known solution‐phase behavior of the studied protein assembly and have therefore been directly used to probe the protein assembly topology and stability as a function of ionic strength and ph.