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Kinetic evidence of an on‐pathway intermediate in the folding of lysozyme
Author(s) -
Bai Yawen
Publication year - 2000
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.9.1.194
Subject(s) - folding (dsp implementation) , protein folding , lysozyme , biophysics , chemistry , crystallography , physics , biology , biochemistry , electrical engineering , engineering
By means of a kinetic test, it was demonstrated that one of the folding intermediates ( I α ) of hen lysozyme with α‐domain folded and β‐domain unfolded is on the folding pathway under the classical definition. l α folds to the native ( N ) state directly (unfolded ( U ) ⟺ I α ⟺ N ) without having to unfold to U and then refold to N through alternative folding pathways as in I α ⟺ U ⟺ N .