Premium
Energy landscape of a peptide consisting of α‐helix, 3 10 ‐helix, β‐turn, β‐hairpin, and other disordered conformations
Author(s) -
Higo Junichi,
Ito Nobutoshi,
Kuroda Masataka,
Ono Satoshi,
Nakajima Nobuyuki,
Nakamura Haruki
Publication year - 2001
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.44901
Subject(s) - random coil , peptide , helix (gastropod) , energy landscape , turn (biochemistry) , chemistry , molecular dynamics , alpha helix , crystallography , peptide conformation , protein structure , protein secondary structure , physics , circular dichroism , computational chemistry , biology , biochemistry , ecology , snail
The energy landscape of a peptide [Ace‐Lys‐Gln‐Cys‐Arg‐Glu‐Arg‐Ala‐Nme] in explicit water was studied with a multicanonical molecular dynamics simulation, and the AMBER parm96 force field was used for the energy calculation. The peptide was taken from the recognition helix of the DNA‐binding protein, c‐Myb. A rugged energy landscape was obtained, in which the random‐coil conformations were dominant at room temperature. The CD spectra of the synthesized peptide revealed that it is in the random state at room temperature. However, the 300 K canonical ensemble, Q(300K) , contained α‐helix, 3 10 ‐helix, β‐turn, and β‐hairpin structures with small but notable probabilities of existence. The complete α‐helix, imperfect α‐helix, and random‐coil conformations were separated from one another in the conformational space. This means that the peptide must overcome energy barriers to form the α‐helix. The overcoming process may correspond to the hydrogen‐bond rearrangements from peptide–water to peptide–peptide interactions. The β‐turn, imperfect 3 10 ‐helix, and β‐hairpin structures, among which there are no energy barriers at 300 K, were embedded in the ensemble of the random‐coil conformations. Two types of β‐hairpin with different β‐turn regions were observed in Q(300K) . The two β‐hairpin structures may have different mechanisms for the β‐hairpin formation. The current study proposes a scheme that the random state of this peptide consists of both ordered and disordered conformations. In contrast, the energy landscape obtained from the parm94 force field was funnel like, in which the peptide formed the helical conformation at room temperature and random coil at high temperature.