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Structure determinants of substrate specificity of hydroxynitrile lyase from Manihot esculenta
Author(s) -
Lauble Hanspeter,
Miehlich Burkhard,
Förster Siegfried,
Kobler Christoph,
Wajant Harald,
Effenberger Franz
Publication year - 2002
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.33702
Subject(s) - active site , chemistry , lyase , substrate (aquarium) , stereochemistry , active center , alanine , enzyme , hydrolase , biochemistry , amino acid , biology , ecology
Tryptophan 128 of hydroxynitrile lyase of Manihot esculenta (MeHNL) covers a significant part of a hydrophobic channel that gives access to the active site of the enzyme. This residue was therefore substituted in the mutant MeHNL‐W128A by alanine to study its importance for the substrate specificity of the enzyme. Wild‐type MeHNL and MeHNL‐W128A showed comparable activity on the natural substrate acetone cyanohydrin (53 and 40 U/mg, respectively). However, the specific activities of MeHNL‐W128A for the unnatural substrates mandelonitrile and 4‐hydroxymandelonitrile are increased 9‐fold and ∼450‐fold, respectively, compared with the wild‐type MeHNL. The crystal structure of the MeHNL‐W128A substrate‐free form at 2.1 Å resolution indicates that the W128A substitution has significantly enlarged the active‐site channel entrance, and thereby explains the observed changes in substrate specificity for bulky substrates. Surprisingly, the MeHNL‐W128A–4‐hydroxybenzaldehyde complex structure at 2.1 Å resolution shows the presence of two hydroxybenzaldehyde molecules in a sandwich type arrangement in the active site with an additional hydrogen bridge to the reacting center.