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Crystal structure of an anti‐interleukin‐2 monoclonal antibody Fab complexed with an antigenic nonapeptide
Author(s) -
Afonin Pavel V.,
Fokin Andrey V.,
Tsygannik Igor N.,
Mikhailova Irina YU.,
Onoprienko Lyudmila V.,
Mikhaleva Inna I.,
Ivanov Vadim T.,
Mareeva Tat'yana YU.,
Nesmeyanov Vladimir A.,
Li Naiyin,
Pangborn Walter A.,
Duax William L.,
Pletnev Vladimir Z.
Publication year - 2001
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.3101
Subject(s) - epitope , chemistry , immunoglobulin fab fragments , peptide , monoclonal antibody , antigen , complementarity determining region , antibody , stereochemistry , binding site , linear epitope , microbiology and biotechnology , biochemistry , biology , immunology
The three‐dimensional structure of the Fab fragment of a monoclonal antibody (LNKB‐2) to human interleukin‐2 (IL‐2) complexed with a synthetic antigenic nonapeptide, Ac‐Lys‐Pro‐Leu‐Glu‐Glu‐Val‐Leu‐Asn‐Leu‐OMe, has been determined at 3.0 Å resolution. In the structure, four out of the six hypervariable loops of the Fab (complementarity determining regions [CDRs] L1, H1, H2, and H3) are involved in peptide association through hydrogen bonding, salt bridge formation, and hydrophobic interactions. The Tyr residues in the Fab antigen binding site play a major role in antigen–antibody recognition. The structures of the complexed and uncomplexed Fab were compared. In the antigen binding site the CDR‐L1 loop of the antibody shows the largest structural changes upon peptide binding. The peptide adopts a mostly α‐helical conformation similar to that in the epitope fragment 64–72 of the IL‐2 antigen. The side chains of residues Leu 66, Val 69, and Leu 70, which are shielded internally in the IL‐2 structure, are involved in interactions with the Fab in the complex studied. This indicates that antibody–antigen complexation involves a significant rearrangement of the epitope‐containing region of the IL‐2 with retention of the α‐helical character of the epitope fragment.