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Disulfide structure of alfimeprase: A recombinant analog of fibrolase
Author(s) -
Jones Geoff,
Ronk Michael,
Mori Faith,
Zhang Zhongqi
Publication year - 2001
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.110101
Subject(s) - edman degradation , disulfide bond , recombinant dna , chemistry , mass spectrometry , peptide , residue (chemistry) , cysteine , stereochemistry , peptide sequence , biochemistry , chromatography , enzyme , gene
The disulfide structure of alfimeprase, a recombinant analog of fibrolase, was experimentally determined by a combination of peptide mapping, Edman degradation, and mass spectrometry. The three disulfide bonds were determined to be Cys‐116/196, Cys‐156 /180, and Cys‐158/163 with the residue number system of alfimeprase.