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Crystal structure of AGR_C_4470p from Agrobacterium tumefaciens
Author(s) -
Vorobiev Sergey M.,
Neely Helen,
Seetharaman Jayaraman,
Ma LiChung,
Xiao Rong,
Acton Thomas B.,
Montelione Gaetano T.,
Tong Liang
Publication year - 2007
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.062663307
Subject(s) - agrobacterium tumefaciens , escherichia coli , biology , plasmid , homology (biology) , homology modeling , heme , ti plasmid , dimer , gene , agrobacterium , protein subunit , microbiology and biotechnology , genetics , chemistry , biochemistry , transgene , organic chemistry , enzyme
We report here the crystal structure at 2.0 Å resolution of the AGR_C_4470p protein from the Gram‐negative bacterium Agrobacterium tumefaciens . The protein is a tightly associated dimer, each subunit of which bears strong structural homology with the two domains of the heme utilization protein ChuS from Escherichia coli and HemS from Yersinia enterocolitica . Remarkably, the organization of the AGR_C_4470p dimer is the same as that of the two domains in ChuS and HemS, providing structural evidence that these two proteins evolved by gene duplication. However, the binding site for heme, while conserved in HemS and ChuS, is not conserved in AGR_C_4470p, suggesting that it probably has a different function. This is supported by the presence of two homologs of AGR_C_4470p in E. coli , in addition to the ChuS protein.