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Enhanced cell‐free protein expression by fusion with immunoglobulin Cκ domain
Author(s) -
Palmer Elizabeth,
Liu Hong,
Khan Farid,
Taussig Michael J.,
He Mingyue
Publication year - 2006
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.062429906
Subject(s) - fusion protein , cell free protein synthesis , fusion , escherichia coli , cell , immunoglobulin light chain , antibody , chemistry , immunoglobulin domain , microbiology and biotechnology , cell fusion , computational biology , biology , biochemistry , protein biosynthesis , gene , recombinant dna , genetics , linguistics , philosophy
Abstract While cell‐free systems are increasingly used for protein expression in structural and functional studies, several proteins are difficult to express or expressed only at low levels in cell‐free lysates. Here, we report that fusion of the human immunoglobulin κ light chain constant domain (Cκ) at the C terminus of four representative proteins dramatically improved their production in the Escherichia coli S30 system, suggesting that enhancement of cell‐free protein expression by Cκ fusion will be widely applicable.