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New Escherichia coli outer membrane proteins identified through prediction and experimental verification
Author(s) -
Marani Paola,
Wagner Samuel,
Baars Louise,
Genevaux Pierre,
De Gier JanWillem,
Nilsson Ingmarie,
Casadio Rita,
Von Heijne Gunnar
Publication year - 2006
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.051889506
Subject(s) - bacterial outer membrane , escherichia coli , membrane protein , proteomics , biology , membrane , microbiology and biotechnology , chemistry , biochemistry , computational biology , biophysics , gene
Many new Escherichia coli outer membrane proteins have recently been identified by proteomics techniques. However, poorly expressed proteins and proteins expressed only under certain conditions may escape detection when wild‐type cells are grown under standard conditions. Here, we have taken a complementary approach where candidate outer membrane proteins have been identified by bioinformatics prediction, cloned and overexpressed, and finally localized by cell fractionation experiments. Out of eight predicted outer membrane proteins, we have confirmed the outer membrane localization for five—YftM, YaiO, YfaZ, CsgF, and YliI—and also provide preliminary data indicating that a sixth—YfaL—may be an outer membrane autotransporter.