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Gateway vectors for the production of combinatorially‐tagged His 6 ‐MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli
Author(s) -
Nallamsetty Sreedevi,
Austin Brian P.,
Penrose Kerri J.,
Waugh David S.
Publication year - 2005
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.051718605
Subject(s) - periplasmic space , maltose binding protein , escherichia coli , cytoplasm , fusion protein , biochemistry , biology , tobacco etch virus , chemistry , recombinant dna , genetics , gene , plant virus , virus , potyvirus
Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purification. To compensate for this shortcoming, we have engineered and successfully tested Gateway destination vectors for the production of dual His 6 MBP‐tagged fusion proteins in the cytoplasm and periplasm of E. coli . The MBP moiety improves the yield and solubility of its fusion partners while the hexahistidine tag (His‐tag) serves to facilitate their purification. The availability of a vector that targets His 6 MBP fusion proteins to the periplasm expands the utility of this dual tagging approach to include proteins that contain disulfide bonds or are toxic in the bacterial cytoplasm.