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Novel surfactant mixtures for NMR spectroscopy of encapsulated proteins dissolved in low‐viscosity fluids
Author(s) -
Peterson Ronald W.,
Pometun Maxim S.,
Shi Zhengshuang,
Wand A. Joshua
Publication year - 2005
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.051535405
Subject(s) - pulmonary surfactant , micelle , chemistry , aqueous solution , nuclear magnetic resonance spectroscopy , chemical engineering , viscosity , spectroscopy , chemical physics , materials science , organic chemistry , biochemistry , physics , quantum mechanics , engineering , composite material
NMR spectroscopy of encapsulated proteins dissolved in low‐viscosity fluids is emerging as a tool for biophysical studies of proteins in atomic detail in a variety of otherwise inaccessible contexts. The central element of the approach is the encapsulation of the protein of interest within the aqueous core of a reverse micelle with high structural fidelity. The process of encapsulation is highly dependent upon the nature of the surfactant(s) employed. Here we describe novel mixtures of surfactants that are capable of successfully encapsulating a range of types of proteins under a variety of conditions.