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Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
Author(s) -
García Javier,
Puentes Alvaro,
Rodríguez Luis,
Ocampo Marisol,
Curtidor Hernando,
Vera Ricardo,
Lopez Ramses,
Valbuena John,
Cortes Jimena,
Vanegas Magnolia,
Barrero Carlos,
Patarroyo Manuel A.,
Urquiza Mauricio,
Patarroyo Manuel E.
Publication year - 2005
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.051526305
Subject(s) - mycobacterium tuberculosis , peptide , peptide sequence , biology , microbiology and biotechnology , antibody , membrane protein , mycobacterium bovis , tuberculosis , u937 cell , biochemistry , binding protein , cell , gene , chemistry , membrane , in vitro , immunology , medicine , pathology
The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT‐PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25‐kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20‐amino‐acid‐long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 ( 161 DVFSAVRADDSPTGEMQVAQY 180 ) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross‐linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.