Premium
Rv0216, a conserved hypothetical protein from Mycobacterium tuberculosis that is essential for bacterial survival during infection, has a double hotdog fold
Author(s) -
Castell Alina,
Johansson Patrik,
Unge Torsten,
Jones T. Alwyn,
Bäckbro Kristina
Publication year - 2005
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.051442305
Subject(s) - mycobacterium tuberculosis , biology , tuberculosis , bacteria , gene , genome , hypothetical protein , microbiology and biotechnology , protein structure , conserved sequence , function (biology) , computational biology , genetics , biochemistry , peptide sequence , medicine , pathology
The Mycobacterium tuberculosis genome contains about 4000 genes, of which approximately a third code for proteins of unknown function or are classified as conserved hypothetical proteins. We have determined the three‐dimensional structure of one of these, the rv0216 gene product, which has been shown to be essential for M. tuberculosis growth in vivo. The structure exhibits the greatest similarity to bacterial and eukaryotic hydratases that catalyse the R‐specific hydration of 2‐enoyl coenzyme A. However, only part of the catalytic machinery is conserved in Rv0216 and it showed no activity for the substrate crotonyl‐CoA. The structure of Rv0216 allows us to assign new functional annotations to a family of seven other M. tuberculosis proteins, a number if which are essential for bacterial survival during infection and growth.