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Comparison of crystal structures of human type 3 3α‐hydroxysteroid dehydrogenase reveals an “induced‐fit” mechanism and a conserved basic motif involved in the binding of androgen
Author(s) -
Couture JeanFrançois,
Pereira De JésusTran Karine,
Roy AnneMarie,
Cantin Line,
Côté PierreLuc,
Legrand Pierre,
LuuThe Van,
Labrie Fernand,
Breton Rock
Publication year - 2005
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.051353205
Subject(s) - chemistry , oxidoreductase , binding site , steroid , stereochemistry , dihydrotestosterone , hydroxysteroid dehydrogenase , dehydrogenase , biochemistry , enzyme , androgen , hormone
Abstract The aldo‐keto reductase (AKR) human type 3 3α‐hydroxysteroid dehydrogenase (h3α–HSD3, AKR1C2) plays a crucial role in the regulation of the intracellular concentrations of testosterone and 5α‐dihydrotestosterone (5α‐DHT), two steroids directly linked to the etiology and the progression of many prostate diseases and cancer. This enzyme also binds many structurally different molecules such as 4‐hydroxynonenal, polycyclic aromatic hydrocarbons, and indanone. To understand the mechanism underlying the plasticity of its substrate‐binding site, we solved the binary complex structure of h3α–HSD3‐NADP(H) at 1.9 Å resolution. During the refinement process, we found acetate and citrate molecules deeply engulfed in the steroid‐binding cavity. Superimposition of this structure with the h3α–HSD3‐NADP(H)‐testosterone/acetate ternary complex structure reveals that one of themobile loops forming the binding cavity operates a slight contraction movement against the citrate molecule while the side chains of many residues undergo numerous conformational changes, probably to create an optimal binding site for the citrate. These structural changes, which altogether cause a reduction of the substrate‐binding cavity volume (from 776 Å 3 in the presence of testosterone/acetate to 704 Å 3 in the acetate/citratecomplex), are reminiscent of the “induced‐fit” mechanism previously proposed for the aldose reductase, another member of the AKR superfamily. We also found that the replacement of residues Arg 301 and Arg 304 , localized near the steroid‐binding cavity, significantly affects the 3α–HSD activity of this enzyme toward 5α‐DHT and completely abolishes its 17β–HSD activity on 4‐dione. All these results have thus been used to reevaluate the binding mode of this enzyme for androgens.