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Experimentally based topology models for E. coli inner membrane proteins
Author(s) -
Rapp Mikaela,
Drew David,
Daley Daniel O.,
Nilsson Johan,
Carvalho Tiago,
Melén Karin,
De Gier JanWillem,
Von Heijne Gunnar
Publication year - 2004
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.03553804
Subject(s) - topology (electrical circuits) , periplasmic space , membrane topology , membrane protein , inner membrane , escherichia coli , chemistry , biology , membrane , gene , biochemistry , mathematics , combinatorics
Membrane protein topology predictions can be markedly improved by the inclusion of even very limited experimental information. We have recently introduced an approach for the production of reliable topology models based on a combination of experimental determination of the location (cytoplasmic or periplasmic) of a protein's C terminus and topology prediction. Here, we show that determination of the location of a protein's C terminus, rather than some internal loop, is the best strategy for large‐scale topology mapping studies. We further report experimentally based topology models for 31 Escherichia coli inner membrane proteins, using methodology suitable for genome‐scale studies.