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Conformational analysis of HAMLET, the folding variant of human α‐lactalbumin associated with apoptosis
Author(s) -
Casbarra Annarita,
Birolo Leila,
Infusini Giuseppe,
Dal Piaz Fabrizio,
Svensson Malin,
Pucci Piero,
Svanborg Catharina,
Marino Gennaro
Publication year - 2004
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.03474704
Subject(s) - hamlet (protein complex) , chemistry , hydrogen–deuterium exchange , folding (dsp implementation) , lactalbumin , alpha lactalbumin , proteases , protein structure , stereochemistry , biophysics , crystallography , biochemistry , mass spectrometry , biology , enzyme , chromatography , genetics , electrical engineering , engineering
A combination of hydrogen/deuterium (H/D) exchange and limited proteolysis experiments coupled to mass spectrometry analysis was used to depict the conformation in solution of HAMLET, the folding variant of human α‐lactalbumin, complexed to oleic acid, that induces apoptosis in tumor and immature cells. Although near‐ and far‐UV CD and fluorescence spectroscopy were not able to discriminate between HAMLET and apo‐α‐lactalbumin, H/D exchange experiments clearly showed that they correspond to two distinct conformational states, with HAMLET incorporating a greater number of deuterium atoms than the apo and holo forms. Complementary proteolysis experiments revealed that HAMLET and apo are both accessible to proteases in the β‐domain but showed substantial differences in accessibility to proteases at specific sites. The overall results indicated that the conformational changes associated with the release of Ca 2+ are not sufficient to induce the HAMLET conformation. Metal depletion might represent the first event to produce a partial unfolding in the β‐domain of α‐lactalbumin, but some more unfolding is needed to generate the active conformation HAMLET, very likely allowing the protein to bind the C18:1 fatty acid moiety. On the basis of these data, a putative binding site of the oleic acid, which stabilizes the HAMLET conformation, is proposed.