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The solution structure of the pH‐induced monomer of dynein light‐chain LC8 from Drosophila
Author(s) -
Makokha Moses,
Huang Yuanpeng Janet,
Montelione Gaetano,
Edison Arthur S.,
Barbar Elisar
Publication year - 2004
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1110/ps.03462204
Subject(s) - dimer , monomer , chemistry , crystallography , nuclear magnetic resonance spectroscopy , dynein , biophysics , stereochemistry , polymer , biology , microtubule , organic chemistry , microbiology and biotechnology
The structure of Drosophila LC8 pH‐induced monomer has been determined by NMR spectroscopy using the program AutoStructure. The structure at pH 3 and 30°C is similar to the individual subunits of mammalian LC8 dimer with the exception that a β strand, which crosses between monomers to form an intersubunit β‐sheet in the dimer, is a flexible loop with turnlike conformations in the monomer. Increased flexibility in the interface region relative to the rest of the protein is confirmed by dynamic measurements based on 15 N relaxation. Comparison of the monomer and dimer structures indicates that LC8 is not a domain swapped dimer.