The solution structure of the pH‐induced monomer of dynein light‐chain LC8 from  Drosophila | Zendy

Makokha Moses | Zendy; Huang Yuanpeng Janet | Zendy; Montelione Gaetano | Zendy; Edison Arthur S. | Zendy; Barbar Elisar | Zendy

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The solution structure of the pH‐induced monomer of dynein light‐chain LC8 from Drosophila

Author(s) -

Makokha Moses,

Huang Yuanpeng Janet,

Montelione Gaetano,

Edison Arthur S.,

Barbar Elisar

Publication year - 2004

Publication title -

protein science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.353

H-Index - 175

eISSN - 1469-896X

pISSN - 0961-8368

DOI - 10.1110/ps.03462204

Subject(s) - dimer , monomer , chemistry , crystallography , nuclear magnetic resonance spectroscopy , dynein , biophysics , stereochemistry , polymer , biology , microtubule , organic chemistry , microbiology and biotechnology

The structure of Drosophila LC8 pH‐induced monomer has been determined by NMR spectroscopy using the program AutoStructure. The structure at pH 3 and 30°C is similar to the individual subunits of mammalian LC8 dimer with the exception that a β strand, which crosses between monomers to form an intersubunit β‐sheet in the dimer, is a flexible loop with turnlike conformations in the monomer. Increased flexibility in the interface region relative to the rest of the protein is confirmed by dynamic measurements based on 15 N relaxation. Comparison of the monomer and dimer structures indicates that LC8 is not a domain swapped dimer.

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