Open AccessInfluence of cell line and cell cycle phase on sonoporation transfection efficiency in cervical carcinoma cells under the same physical conditions
Author(s) -
Samuel Pichardo,
Melissa Togtema,
Robert Jackson,
Ingeborg Zehbe,
Laura Curiel
Publication year - 2013
Publication title -
ieee transactions on ultrasonics, ferroelectrics, and frequency control
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.159
H-Index - 136
eISSN - 1525-8955
pISSN - 0885-3010
DOI - 10.1109/tuffc.2013.2581
Subject(s) - fields, waves and electromagnetics
Using cervical-carcinoma-derived cells as a model, the present study investigates the effects cell line and cell cycle phase have on sonoporation transfection efficiency under the same physical conditions. A plasmid expressing green fluorescent protein (GFP) was used to measure transfection efficiency. To evaluate the effect of cell type, CaSki, HeLa, and SiHa cells were sonoporated using an acoustic pressure of 1 MPa for 30 s with a duty cycle of 4.8% in the presence of the GFP plasmid. To study the effect of cell cycle phase, SiHa cells were synchronized at S-phase using a double thymidine block and sonoporated at different time points after the block. Contrast agent microbubbles were used at a 0.33% volume concentration. Results indicated that both cell line and cell cycle phase impact the transfection efficiency obtained with sonoporation.