Open AccessSolid-Phase Isothermal DNA Amplification and Detection on Quartz Crystal Microbalance Using Liposomes and Dissipation Monitoring
Author(s) -
Aristea Grammoustianou,
George Papadakis,
Electra Gizeli
Publication year - 2017
Publication title -
ieee sensors letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.382
H-Index - 10
ISSN - 2475-1472
DOI - 10.1109/lsens.2017.2739803
Subject(s) - components, circuits, devices and systems , robotics and control systems , communication, networking and broadcast technologies , signal processing and analysis
In our sensing configuration, the quartz crystal microbalance device is employed for the first time for the solid phase amplification via the recombinase polymerase amplification method and simultaneous detection of DNA amplicons on the device surface. For amplicon detection, a novel methodology is described, where the formation of surface-bound DNA amplicons is detected by using liposomes as an amplification probe and by selectively monitoring the dissipation change during liposome binding. The presented methodology possesses several advantages over existing methods, i.e., it is fast, achieving the detection of DNA produced after only 5 min of amplification, and simple, since it does not require any post-amplification DNA extraction step.