Open AccessEnhanced Detection of Single Viruses On-Chip via Hydrodynamic Focusing
Author(s) -
Jennifer A. Black,
Erik Hamilton,
Raúl A. Reyes Hueros,
Joshua W. Parks,
Aaron R. Hawkins,
Holger Schmidt
Publication year - 2019
Publication title -
ieee journal of selected topics in quantum electronics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.131
H-Index - 159
eISSN - 1558-4542
pISSN - 1077-260X
DOI - 10.1109/jstqe.2018.2854574
Subject(s) - optofluidics , microfluidics , materials science , chip , planar , lab on a chip , nanotechnology , silicon , rapid prototyping , optoelectronics , computer science , telecommunications , computer graphics (images) , composite material
Planar optofluidics provide a powerful tool for facilitating chip-scale light-matter interactions. Silicon-based liquid core waveguides have been shown to offer single molecule sensitivity for efficient detection of bioparticles. Recently, a PDMS based planar optofluidic platform was introduced that opens the way to rapid development and prototyping of unique structures, taking advantage of the positive attributes of silicon dioxide-based optofluidics and PDMS based microfluidics. Here, hydrodynamic focusing is integrated into a PDMS based optofluidic chip to enhance the detection of single H1N1 viruses on-chip. Chip-plane focusing is provided by a system of microfluidic channels to force the particles towards a region of high optical collection efficiency. Focusing is demonstrated and enhanced detection is quantified using fluorescent polystyrene beads where the coefficient of variation is found to decrease by a factor of 4 with the addition of hydrodynamic focusing. The mean signal amplitude of fluorescently tagged single H1N1 viruses is found to increase with the addition of focusing by a factor of 1.64.