Open AccessOptical Molecular Imaging and Spectroscopy of Oxygenation and Metabolism in Tumors
Author(s) -
Jay Im,
Narasimhan Rajaram
Publication year - 2016
Publication title -
ieee journal of selected topics in quantum electronics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.131
H-Index - 159
eISSN - 1558-4542
pISSN - 1077-260X
DOI - 10.1109/jstqe.2015.2501379
Subject(s) - engineered materials, dielectrics and plasmas , photonics and electrooptics
There is growing evidence that aerobic glycolysis provides tumors with a growth advantage and could play a role in promoting an aggressive tumor phenotype. Quantitative visualization of aerobic glycolysis within tumors could provide a prognostic marker of long-term tumor outcome. We have developed a method for simultaneous imaging of vascular oxygenation and metabolism in tumors using high resolution intravital microscopy with the goal of visualizing and quantifying the extent of aerobic glycolysis within a tumor. As part of this review, we detail our efforts toward deriving the quantitative parameters related to tumor glycolytic demand using a fluorescent glucose analog called 2-NBDG, and quantifying the relationship between vascular oxygenation and glycolytic demand. Finally, we present our recent results demonstrating the ability of diffuse optical spectroscopy to measure both oxygenation and glycolytic demand in murine models of cancer.