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Structural analysis of glycoproteins: building N‐linked glycans with Coot
Author(s) -
Emsley Paul,
Crispin Max
Publication year - 2018
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s2059798318005119
Subject(s) - glycan , glycoprotein , graphics , computer science , computational biology , biology , computer graphics (images) , genetics
Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level. The model‐building tools of Coot have been combined and extended to assist or automate the building of N‐linked glycans. The model is built by the addition of monosaccharides, placed by variation of internal coordinates. The subsequent model is refined by real‐space refinement, which is stabilized with modified and additional restraints. It is hoped that these enhanced building tools will help to reduce building errors of N‐linked glycans and improve our knowledge of the structures of glycoproteins.