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Nogo Receptor crystal structures with a native disulfide pattern suggest a novel mode of self‐interaction
Author(s) -
Pronker Matti F.,
Tas Roderick P.,
Vlieg Hedwich C.,
Janssen Bert J. C.
Publication year - 2017
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s2059798317013791
Subject(s) - leucine rich repeat , receptor , chemistry , glycoprotein , biophysics , myelin , microbiology and biotechnology , crystallography , biochemistry , biology , neuroscience , central nervous system
The Nogo Receptor (NgR) is a glycophosphatidylinositol‐anchored cell‐surface protein and is a receptor for three myelin‐associated inhibitors of regeneration: myelin‐associated glycoprotein, Nogo66 and oligodendrocyte myelin glycoprotein. In combination with different co‐receptors, NgR mediates signalling that reduces neuronal plasticity. The available structures of the NgR ligand‐binding leucine‐rich repeat (LRR) domain have an artificial disulfide pattern owing to truncated C‐terminal construct boundaries. NgR has previously been shown to self‐associate via its LRR domain, but the structural basis of this interaction remains elusive. Here, crystal structures of the NgR LRR with a longer C‐terminal segment and a native disulfide pattern are presented. An additional C‐terminal loop proximal to the C‐terminal LRR cap is stabilized by two newly formed disulfide bonds, but is otherwise mostly unstructured in the absence of any stabilizing interactions. NgR crystallized in six unique crystal forms, three of which share a crystal‐packing interface. NgR crystal‐packing interfaces from all eight unique crystal forms are compared in order to explore how NgR could self‐interact on the neuronal plasma membrane.