Low‐dose fixed‐target serial synchrotron crystallography

The development of serial crystallography has been driven by the sample requirements imposed by X‐ray free‐electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed‐target approach to high‐throughput serial sampling, it is demonstrated that high‐quality data can be collected from myoglobin crystals, allowing room‐temperature, low‐dose structure determination. The combination of fixed‐target arrays and a fast, accurate translation system allows high‐throughput serial data collection at high hit rates and with low sample consumption.