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Low‐dose fixed‐target serial synchrotron crystallography
Author(s) -
Owen Robin L.,
Axford Danny,
Sherrell Darren A.,
Kuo Anling,
Ernst Oliver P.,
Schulz Eike C.,
Miller R. J. Dwayne,
Mueller-Werkmeister Henrike M.
Publication year - 2017
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s2059798317002996
Subject(s) - throughput , synchrotron , sample (material) , computer science , translation (biology) , serial communication , computer hardware , materials science , optics , chemistry , physics , telecommunications , biochemistry , chromatography , messenger rna , wireless , gene
The development of serial crystallography has been driven by the sample requirements imposed by X‐ray free‐electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed‐target approach to high‐throughput serial sampling, it is demonstrated that high‐quality data can be collected from myoglobin crystals, allowing room‐temperature, low‐dose structure determination. The combination of fixed‐target arrays and a fast, accurate translation system allows high‐throughput serial data collection at high hit rates and with low sample consumption.