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Structural and functional studies of the glycoside hydrolase family 3 β‐glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii
Author(s) -
Gudmundsson Mikael,
Hansson Henrik,
Karkehabadi Saeid,
Larsson Anna,
Stals Ingeborg,
Kim Steve,
Sunux Sergio,
Fujdala Meredith,
Larenas Edmund,
Kaper Thijs,
Sandgren Mats
Publication year - 2016
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s2059798316008482
Subject(s) - hypocrea , glycoside hydrolase , thermophile , cellulase , hydrolysis , chemistry , biochemistry , hydrolase , glycoside , glycosidic bond , glucosidases , beta glucosidase , enzyme , stereochemistry , trichoderma reesei
The filamentous fungus Hypocrea jecorina produces a number of cellulases and hemicellulases that act in a concerted fashion on biomass and degrade it into monomeric or oligomeric sugars. β‐Glucosidases are involved in the last step of the degradation of cellulosic biomass and hydrolyse the β‐glycosidic linkage between two adjacent molecules in dimers and oligomers of glucose. In this study, it is shown that substituting the β‐glucosidase from H. jecorina ( Hj Cel3A) with the β‐glucosidase Cel3A from the thermophilic fungus Rasamsonia emersonii ( Re Cel3A) in enzyme mixtures results in increased efficiency in the saccharification of lignocellulosic materials. Biochemical characterization of Re Cel3A, heterologously produced in H. jecorina , reveals a preference for disaccharide substrates over longer gluco‐oligosaccharides. Crystallographic studies of Re Cel3A revealed a highly N‐glycosylated three‐domain dimeric protein, as has been observed previously for glycoside hydrolase family 3 β‐glucosidases. The increased thermal stability and saccharification yield and the superior biochemical characteristics of Re Cel3A compared with Hj Cel3A and mixtures containing Hj Cel3A make Re Cel3A an excellent candidate for addition to enzyme mixtures designed to operate at higher temperatures.