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Facilitating best practices in collecting anomalous scattering data for de novo structure solution at the ESRF Structural Biology Beamlines
Author(s) -
de Sanctis Daniele,
Oscarsson Marcus,
Popov Alexander,
Svensson Olof,
Leonard Gordon
Publication year - 2016
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s2059798316001042
Subject(s) - phaser , anomalous scattering , structural biology , computer science , synchrotron , computational science , computational biology , diffraction , biology , physics , optics , biochemistry
The constant evolution of synchrotron structural biology beamlines, the viability of screening protein crystals for a wide range of heavy‐atom derivatives, the advent of efficient protein labelling and the availability of automatic data‐processing and structure‐solution pipelines have combined to make de novo structure solution in macromolecular crystallography a less arduous task. Nevertheless, the collection of diffraction data of sufficient quality for experimental phasing is still a difficult and crucial step. Here, some examples of good data‐collection practice for projects requiring experimental phasing are presented and recent developments at the ESRF Structural Biology beamlines that have facilitated these are illustrated.