Premium
Radiation damage and derivatization in macromolecular crystallography: a structure factor's perspective
Author(s) -
Owen Robin L.,
Sherrell Darren A.
Publication year - 2016
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.374
H-Index - 138
ISSN - 2059-7983
DOI - 10.1107/s2059798315021555
Subject(s) - derivatization , radiation damage , macromolecule , radiation , chemistry , biophysics , chromatography , physics , optics , biology , biochemistry , mass spectrometry
During, or even after, data collection the presence and effects of radiation damage in macromolecular crystallography may not always be immediately obvious. Despite this, radiation damage is almost always present, with site‐specific damage occurring on very short time (dose) scales well before global damage becomes apparent. A result of both site‐specific radiation damage and derivatization is a change in the relative intensity of reflections. The size and approximate rate of onset of X‐ray‐induced transformations is compared with the changes expected from derivatization, and strategies for minimizing radiation damage are discussed.