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Three‐dimensional single‐cell imaging with X‐ray waveguides in the holographic regime
Author(s) -
Krenkel Martin,
Toepperwien Mareike,
Alves Frauke,
Salditt Tim
Publication year - 2017
Publication title -
acta crystallographica section a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.742
H-Index - 83
ISSN - 2053-2733
DOI - 10.1107/s2053273317007902
Subject(s) - holography , phase retrieval , bottleneck , computer science , optics , phase (matter) , physics , algorithm , quantum mechanics , fourier transform , embedded system
X‐ray tomography at the level of single biological cells is possible in a low‐dose regime, based on full‐field holographic recordings, with phase contrast originating from free‐space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi‐point sources provided by X‐ray waveguides, here this approach is extended in several ways. First, the phase‐retrieval algorithms are extended by an optimized deterministic inversion, based on a multi‐distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single‐distance and multi‐distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data.