Premium
Structural characterization of three noncanonical NTF2‐like superfamily proteins: implications for polyketide biosynthesis
Author(s) -
Vuksanovic Nemanja,
Zhu Xuechen,
Serrano Dante A.,
Siitonen Vilja,
Metsä-Ketelä Mikko,
Melançon Charles E.,
Silvaggi Nicholas R.
Publication year - 2020
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x20009814
Subject(s) - polyketide , streptomyces coelicolor , biosynthesis , polyketide synthase , biology , myxobacteria , gene cluster , streptomyces , enzyme , function (biology) , biochemistry , genetics , gene , bacteria
Proteins belonging to the NTF2‐like superfamily are present in the biosynthetic pathways of numerous polyketide natural products, such as anthracyclins and benzoisochromanequinones. Some have been found to be bona fide polyketide cyclases, but many of them have roles that are currently unknown. Here, the X‐ray crystal structures of three NTF2‐like proteins of unknown function are reported: those of ActVI‐ORFA from Streptomyces coelicolor A3(2) and its homologs Caci_6494, a protein from an uncharacterized biosynthetic cluster in Catenulispora acidiphila , and Aln2 from Streptomyces sp. CM020, a protein in the biosynthetic pathway of alnumycin. The presence of a solvent‐accessible cavity and the conservation of the His/Asp dyad that is characteristic of many polyketide cyclases suggest a potential enzymatic role for these enzymes in polyketide biosynthesis.