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AMi: a GUI‐based, open‐source system for imaging samples in multi‐well plates
Author(s) -
Bohm Andrew
Publication year - 2019
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x19009853
Subject(s) - python (programming language) , graphical user interface , raspberry pi , microcomputer , arduino , computer science , computer graphics (images) , software , open source , operating system , interface (matter) , computer hardware , embedded system , internet of things , telecommunications , chip , bubble , maximum bubble pressure method
Described here are instructions for building and using an inexpensive automated microscope (AMi) that has been specifically designed for viewing and imaging the contents of multi‐well plates. The X , Y , Z translation stage is controlled through dedicated software ( AMiGUI ) that is being made freely available. Movements are controlled by an Arduino‐based board running grbl , and the graphical user interface and image acquisition are controlled via a Raspberry Pi microcomputer running Python. Images can be written to the Raspberry Pi or to a remote disk. Plates with multiple sample wells at each row/column position are supported, and a script file for automated z ‐stack depth‐of‐field enhancement is written along with the images. The graphical user interface and real‐time imaging also make it easy to manually inspect and capture images of individual samples.