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Structure of Aspergillus aculeatus β‐1,4‐galactanase in complex with galactobiose
Author(s) -
Torpenholt Søs,
Poulsen Jens-Christian N.,
Muderspach Sebastian Jannick,
De Maria Leonardo,
Lo Leggio Leila
Publication year - 2019
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x19005612
Subject(s) - aspergillus , biology , microbiology and biotechnology
β‐1,4‐Galactanases are glycoside hydrolases that are involved in the degradation of pectin and belong to family 53 in the classification of glycoside hydrolases. Previous studies have elucidated the structures of several fungal and two bacterial galactanases, while biochemical studies have indicated differences in the product profiles of different members of the family. Structural studies of ligand complexes have to date been limited to the bacterial members of the family. Here, the first structure of a fungal galactanase in complex with a disaccharide is presented. Galactobiose binds to subsites −1 and −2, thus improving our understanding of ligand binding to galactanases.