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Cryo‐EM structure of the Ebola virus nucleoprotein–RNA complex
Author(s) -
Kirchdoerfer Robert N.,
Saphire Erica Ollmann,
Ward Andrew B.
Publication year - 2019
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x19004424
Subject(s) - nucleoprotein , ebola virus , virology , ebolavirus , rna , biology , virus , genetics , gene
Ebola virus is an emerging virus that is capable of causing a deadly disease in humans. Replication, transcription and packaging of the viral genome are carried out by the viral nucleocapsid. The nucleocapsid is a complex of the viral nucleoprotein, RNA and several other viral proteins. The nucleoprotein forms large, RNA‐bound, helical filaments and acts as a scaffold for additional viral proteins. The 3.1 Å resolution single‐particle cryo‐electron microscopy structure of the nucleoprotein–RNA helical filament presented here resembles previous structures determined at lower resolution, while providing improved molecular details of protein–protein and protein–RNA interactions. The higher resolution of the structure presented here will facilitate the design and characterization of novel and specific Ebola virus therapeutics targeting the nucleocapsid.