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Structure of an Influenza A virus N9 neuraminidase with a tetrabrachion‐domain stalk
Author(s) -
Streltsov Victor A.,
Schmidt Peter M.,
McKimm-Breschkin Jennifer L.
Publication year - 2019
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x18017892
Subject(s) - stalk , neuraminidase , homotetramer , virus , virology , biology , transmembrane domain , turnip yellow mosaic virus , chemistry , biochemistry , membrane , gene , plant virus , protein subunit , horticulture
The influenza neuraminidase (NA) is a homotetramer with head, stalk, transmembrane and cytoplasmic regions. The structure of the NA head with a stalk has never been determined. The NA head from an N9 subtype influenza A virus, A/tern/Australia/G70C/1975 (H1N9), was expressed with an artificial stalk derived from the tetrabrachion (TB) tetramerization domain from Staphylothermus marinus . The NA was successfully crystallized both with and without the TB stalk, and the structures were determined to 2.6 and 2.3 Å resolution, respectively. Comparisons of the two NAs with the native N9 NA structure from egg‐grown virus showed that the artificial TB stalk maintained the native NA head structure, supporting previous biological observations.