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The structure and activity of the glutathione reductase from Streptococcus pneumoniae
Author(s) -
Sikanyika Mwilye,
Aragão David,
McDevitt Christopher A.,
Maher Megan J.
Publication year - 2019
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x18016527
Subject(s) - streptococcus pneumoniae , glutathione reductase , glutathione , microbiology and biotechnology , reductase , chemistry , biochemistry , enzyme , biology , glutathione peroxidase , antibiotics
The glutathione reductase (GR) from Streptococcus pneumoniae is a flavoenzyme that catalyzes the reduction of oxidized glutathione (GSSG) to its reduced form (GSH) in the cytoplasm of this bacterium. The maintenance of an intracellular pool of GSH is critical for the detoxification of reactive oxygen and nitrogen species and for intracellular metal tolerance to ions such as zinc. Here, S. pneumoniae GR ( Sp GR) was overexpressed and purified and its crystal structure determined at 2.56 Å resolution. Sp GR shows overall structural similarity to other characterized GRs, with a dimeric structure that includes an antiparallel β‐sheet at the dimer interface. This observation, in conjunction with comparisons with the interface structures of other GR enzymes, allows the classification of these enzymes into three classes. Analyses of the kinetic properties of Sp GR revealed a significantly higher value for K m(GSSG) (231.2 ± 24.7 µ M ) in comparison to other characterized GR enzymes .