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Structure of glyoxysomal malate dehydrogenase (MDH3) from Saccharomyces cerevisiae
Author(s) -
Moriyama Shu,
Nishio Kazuya,
Mizushima Tsunehiro
Publication year - 2018
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x18011895
Subject(s) - malate dehydrogenase , glyoxylate cycle , biochemistry , nad+ kinase , citrate synthase , citric acid cycle , biology , nicotinamide adenine dinucleotide , saccharomyces cerevisiae , enzyme , cytosol , ternary complex , yeast
Malate dehydrogenase (MDH), a carbohydrate and energy metabolism enzyme in eukaryotes, catalyzes the interconversion of malate to oxaloacetate (OAA) in conjunction with that of nicotinamide adenine dinucleotide (NAD + ) to NADH. Three isozymes of MDH have been reported in Saccharomyces cerevisiae : MDH1, MDH2 and MDH3. MDH1 is a mitochondrial enzyme and a member of the tricarboxylic acid cycle, whereas MDH2 is a cytosolic enzyme that functions in the glyoxylate cycle. MDH3 is a glyoxysomal enzyme that is involved in the reoxidation of NADH, which is produced during fatty‐acid β‐oxidation. The affinity of MDH3 for OAA is lower than those of MDH1 and MDH2. Here, the crystal structures of yeast apo MDH3, the MDH3–NAD + complex and the MDH3–NAD + –OAA ternary complex were determined. The structure of the ternary complex suggests that the active‐site loop is in the open conformation, differing from the closed conformations in mitochondrial and cytosolic malate dehydrogenases.