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The CD163 long‐range scavenger receptor cysteine‐rich repeat: expression, purification and X‐ray crystallographic characterization
Author(s) -
Li Rui,
Ma Hongfang,
Jiang Longguang,
Qiao Songlin,
Zhi Yubao,
Huang Mingdong,
Deng Ruiguang,
Zhang Gaiping
Publication year - 2018
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x18005551
Subject(s) - scavenger receptor , receptor , scavenger , chemistry , peg ratio , biochemistry , cd163 , biology , phenotype , gene , cholesterol , lipoprotein , finance , economics , radical
Scavenger receptors (SRs) play critical roles in various physiological and pathological pathways. One of them, CD163, is a multifunctional endocytic receptor and is characterized by a long‐range scavenger receptor cysteine‐rich (SRCR) repeat. However, the structural and functional details of this long‐range SRCR repeat have not yet been elucidated. In this study, the CD163 long‐range SRCR repeat was expressed in Drosophila Schneider 2 cells. The recombinant protein was homogeneous after purification by metal‐affinity, cation‐exchange and size‐exclusion chromatography. Single crystals were obtained using 20% PEG 4000, 0.15 M potassium sodium tartrate tetrahydrate pH 8.5 and diffracted to 3.30 Å resolution. As the first view of a long‐range SRCR repeat, this work lays the structural basis for a deep understanding of SRs and their multiple functions.