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Crystallization via tubing microfluidics permits both in situ and ex situ X‐ray diffraction
Author(s) -
Gerard Charline J. J.,
Ferry Gilles,
Vuillard Laurent M.,
Boutin Jean A.,
Chavas Leonard M. G.,
Huet Tiphaine,
Ferte Nathalie,
Grossier Romain,
Candoni Nadine,
Veesler Stéphane
Publication year - 2017
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x17013826
Subject(s) - in situ , crystallization , diffractometer , diffraction , microfluidics , materials science , x ray crystallography , protein crystallization , crystallography , crystal (programming language) , nanotechnology , chemical engineering , analytical chemistry (journal) , chemistry , chromatography , optics , crystal structure , physics , computer science , engineering , organic chemistry , programming language
A microfluidic platform was used to address the problems of obtaining diffraction‐quality crystals and crystal handling during transfer to the X‐ray diffractometer. Crystallization conditions of a protein of pharmaceutical interest were optimized and X‐ray data were collected both in situ and ex situ .