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Ectodomain of plasmodesmata‐localized protein 5 in Arabidopsis : expression, purification, crystallization and crystallographic analysis
Author(s) -
Wang Xiaocui,
Zhu Peiyan,
Qu Shanshan,
Zhao Jie,
Singh Prashant K.,
Wang Wei
Publication year - 2017
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 37
ISSN - 2053-230X
DOI - 10.1107/s2053230x1701250x
Subject(s) - ectodomain , plasmodesma , arabidopsis , crystallization , chemistry , arabidopsis thaliana , plant cell , crystallography , microbiology and biotechnology , biophysics , biology , cell , biochemistry , receptor , mutant , gene , organic chemistry
Plasmodesmata‐localized protein 5 (PDLP5) is a cysteine‐rich receptor‐like protein which is localized on the plasmodesmata of Arabidopsis thaliana . Overexpression of PDLP5 can reduce the permeability of the plasmodesmata and further affect the cell‐to‐cell movement of viruses and macromolecules in plants. The ectodomain of PDLP5 contains two DUF26 domains; however, the function of these domains is still unknown. Here, the ectodomain of PDLP5 from Arabidopsis was cloned and overexpressed using an insect expression system and was then purified and crystallized. X‐ray diffraction data were collected to 1.90 Å resolution and were indexed in space group P 1, with unit‐cell parameters a = 41.9, b = 48.1, c = 62.2 Å, α = 97.3, β = 103.1, γ = 99.7°. Analysis of the crystal content indicated that there are two molecules in the asymmetric unit, with a Matthews coefficient of 2.51 Å 3 Da −1 and a solvent content of 50.97%.